RNA in-situ hybridization for pathology-based diagnosis of feline infectious peritonitis (FIP): current diagnostics for FIP and comparison to the current gold standard
نویسندگان
چکیده
Feline infectious peritonitis (FIP) is a systemic disease of cats caused by highly pathogenic variant feline coronavirus, or FCoV. Two distinct genotypes FCoV exist (also referred to as serotypes): Type 1 viruses constitute the vast majority FIP cases, while type 2 are responsible for remaining infections. Immunohistochemistry (IHC) currently serves gold standard diagnosis FIP; however, IHC limited variations in sensitivity. RNA situ hybridization (RNA ISH) has an established foothold diagnostics and presents potentially improved method detection FIP. This proof-of-concept study evaluated efficacy ISH probes targeted FCoV, compared using monoclonal antibody 3-70. Formalin-fixed paraffin-embedded tissues from FIP-positive were used ISH, with presence determined chromogenically. tissue slides then their counterparts, based on metrics including staining intensity abundance. Positive was found be both more intense abundant than IHC, suggesting that sensitive FCoV/FIP comparison - finding awaits further validation.
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ژورنال
عنوان ژورنال: Qeios
سال: 2022
ISSN: ['2632-3834']
DOI: https://doi.org/10.32388/nun8kb.2